IDENTIFICATION OF AGAROLYTIC BACTERIA PDF

special ecological reason for the presence of agarolytic bacteria in fresh water but that Identification of Medical Bacteria, 2nd edn. Cam-. Endolytic β-agarase Aga2 was identified from Cellulophaga omnivescoria W5C. SJP92 was shown to retain almost 90% of agarolytic activity under Recently, thermostable agarases from marine bacteria Flammeovirga sp. Abstract: Agarolytic bacteria use agarase to utilize agar as sole source of carbon. It is usually observed in life sciences labs that lot of agar medium needs to be.

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Production of indole, urease, arginine dihydrolase, and accumulation of PHB. The undigested polysaccharides from bacteris previous digest Oxidation and fermentation tests were done in MOF medium as recommended by Leifson 26but without agar. In our laboratory, we have isolated a few agar-softening and agar-liquefying bacterial strains from the southern Chilean coast to characterize their extracellular agarases in an attempt to contribute to our understanding of the basis of agar hydrolysis.

There was no evidence of a signal at Author information Article notes Copyright and License information Disclaimer.

This article has been cited by other articles in PMC. Cleavage of the polysaccharide chains causes agar softening and allows faster evaporation of water, leading to the formation of depressions. Agarase N-1 had a molecular mass of 33 kDa, as determined by a comparison with the mobility of protein standards Fig.

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Phylogeny of the Vibrionaceae and recommendation for two new genera, Listonella and Shewanella. At longer incubation periods the level of agarase decreases, a trend probably due to the presence of proteases.

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An extracellular ixentification was purified to homogeneity in high yield by gel filtration and two steps of ion-exchange chromatography on DEAE-cellulose.

Additional purification of the enzyme was achieved by gel filtration on Sephadex G75 Fig.

Effect of salt concentration on bactedia activity. HPLC analysis of the hydrolysis products of unsubstituted agar generated by agarase from P. Sugano Y, Noma M. The dialyzate was loaded onto a DEAE-cellulose column 10 by 1.

The highest level of agarase was reached during the stationary phase. The purified protein was determined to be homogeneous on the basis of sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and it had a molecular mass of 33 kDa.

Hydrolysis of agar, alginic acid, carboxymethyl-cellulose, esculin, gelatin, and starch. The GenBank accession number for the small subunit of P.

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K m values of 0. Toffanin for the NMR technical support. Agarase activity was determined by the method of Dygert et al.

Proteins were stained with Coomassie brilliant blue Idenification The single DNA band of approximately 1. Agarotriose was the smallest product detected in this system. In both cases the enzyme showed a molecular mass of 16 kDa, indicating an interaction with these resins. The enzyme gave a single band on SDS-polyacrylamide gels Fig.

Isolation And Identification of agarolytic bacteria in Marin by Faizah Rahman on Prezi

Identigication taxonomy of aerobic, gram-negative bacteria associated with oysters and surrounding seawater of the mediterranean coast. An overnight culture of isolated colonies of strain N-1 was prepared in the medium described above and used to inoculate 2 liters of fresh medium containing 0. In addition, strain N-1, unlike the Shewanella spp.

Enzyme hydrolysis of agar and properties of bacterial agarases. Van Hofsteen B, Malmqvist M. To characterize identidication hydrolysis products of agar with the purified enzyme, a solution of 0.